Syndicate Bank — cash issue

I hello sir/mam
I made payment on dled. Nois.ac.in for registration of diploma in elementary teaching cource.
There is payment option through online so used my syndicate bank debit card which a/c no. Is [protected] to make payment.
Rs.4553.10 at 7:02 pm on 28/09/2017 were debited from my account but application status is showing payment not made.in my appliaction form they were saying to made same payment again but what about my allready deducated balance. Sir can u please try solve my problem. I need refund this payment so that i has made payment again my application last date is gonna over so please sir
Kindly try to resolve the problem..
I will be very thankful
Reference number - d09043063
Mob no.[protected]